cd3 microbead kit (Miltenyi Biotec)
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Structured Review
Miltenyi Biotec
cd3 microbead kit
Cd3 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non+human+primates/CD3+MicroBead+Kit%2C+non-human+primate/pmc13616112-376-0-6
Average 93 stars, based on 16 article reviews
Cd3 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non+human+primates/CD3+MicroBead+Kit%2C+non-human+primate/pmc13616112-376-0-6
Average 93 stars, based on 16 article reviews
cd3 microbead kit - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Isolation of Brain Microglia to Study Persistence of Human Immunodeficiency Virus-1"
Article Title: Isolation of Brain Microglia to Study Persistence of Human Immunodeficiency Virus-1
Journal: Journal of visualized experiments : JoVE
doi: 10.3791/71673
Figure Legend Snippet: ( A-C ) Flow cytometry analysis of CD3 + and CD11b + fractions using the indicated antibodies. ( D-F ) Representative images (bottom) show microglia, CD3 + T cells, and splenic myeloid cells 7 days after culture following isolation from the brain and spleen (Scale: 20x). Abbreviations: SMCs = spleen myeloid cells; MG = microglia. Please click here to view a larger version of this figure .
Techniques Used: Isolation, Flow Cytometry, Analysis
Related Articles
Isolation:Article Title: The role of CD4 T cells in the rhesus central nervous system during homeostasis and viral-induced neuroinflammation Article Snippet: Cryopreserved mononuclear cells from rhesus brain were thawed at room temperature, placed in fresh complete media (For splenic cells: RPMI supplemented with 10% HI-FBS, 1% Lglutamine, 1% penicillin-streptomycin; For brain tissue derived cells: DMEM supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37oC. .. Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for Article Title: The role of CD4 T cells in the rhesus central nervous system during homeostasis and viral-induced neuroinflammation Article Snippet: Cryopreserved mononuclear cells from rhesus brain and splenic tissues were thawed at room temperature, placed in fresh complete media (For splenic cells: RPMI supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin; For brain tissue derived cells: DMEM supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37oC. .. Cells were washed in complete media and CD45+ cells were isolated using CD45 microbeads for Article Title: CD4 T cell Responses in the CNS during SIV infection Article Snippet: Cryopreserved mononuclear cells from rhesus brain were thawed at room temperature, placed in fresh complete media (For splenic cells: RPMI supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin; For brain tissue derived cells: DMEM supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C. .. Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for Article Title: CCR7+ CD4 T Cell Immunosurveillance Disrupted in Chronic SIV-Induced Neuroinflammation in Rhesus Brain Article Snippet: Cryopreserved mononuclear cells from rhesus brain and splenic tissues were thawed at room temperature, placed in fresh complete media (For splenic cells: RPMI supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin; For brain tissue derived cells: DMEM supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C. .. Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for Article Title: Deep analysis of CD4 T cells in the rhesus CNS during SIV infection Article Snippet: Cryopreserved mononuclear cells from rhesus brain were thawed, placed in fresh complete media (For splenic cells: RPMI supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin; For brain tissue derived cells: DMEM supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C. .. Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for Gradient Centrifugation:Article Title: Reactivation of latent HIV-1 in vitro using an ethanolic extract from Euphorbia umbellata (Euphorbiaceae) latex Article Snippet: .. PBMCs obtained by density gradient centrifugation were enriched for CD4 + T cells using the CD4 T cell enrichment kit for |
![( A and B ) Peripheral resting CD4 + T cells were isolated from eight PWH on ART and treated for 24 hours with the following compounds: PMA (200 ng/mL), ionomycin (2 µM), SAHA (350 nM), RGFP966 (2.5 µM), mod-RGFP966 (2.5 µM), and citarinostat (5 µM). (A) The cell-associated HIV gag levels were measured by droplet digital PCR (ddPCR), with results shown individually for each donor, including triplicate ddPCR data. (B) A combined summary of the triplicate ddPCR results from all donors ( n = 8) is presented as averages. PMA 200 ng/mL; ionomycin 2 µM; SAHA 350nM; RGFP966 5µM; mod-RGFP966 5µM; citarinostat 5µM. ( C ) Cell-free HIV gag levels in the culture supernatant from three donors treated with 350 nM SAHA and 5 µM citarinostat were measured. ( D ) Brain tissue from NHP or participants with HIV was mechanically dissociated to generate a single-cell suspension, followed by Percoll gradient separation. CD3 + T cells were depleted using a CD3 selection kit. Brain MG were isolated from the CD3-negative fraction by <t>CD11b</t> + selection. Brain MG were cultured ex vivo for 1 to 2 weeks to allow recovery and attachment before being used in the LRA study. ( E ) Brain MG from participants with HIV were treated with DMSO (control), 40 mM NaCr, 350 nM SAHA, or 10 μM RGFP966 ( n = 4). ( F ) In a separate experiment, cells were treated with DMSO or 5 μM citarinostat ( n = 5). ( G ) Brain MG from NHPs were treated with 5 μM citarinostat or DMSO control ( n = 6). After 24 hours, the drugs were removed, and cells were cultured for another 6 days. Cells and culture supernatants were harvested for HIV LTR RNA [(E) and (F)] or SIV RNA (G) measurement by ddPCR. A combined summary of the triplicate ddPCR results is presented as averages. Error bars represent SD; * P < 0.05; ** P < 0.01; *** P < 0.001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8052/pmc13068052/pmc13068052__sciadv.aec0149-f8.jpg)